Expression and clinical significance of serum response factor and myosin heavy chain 9 in esophageal squamous cell carcinoma / 中国综合临床

Objective:To investigate the expression and clinical significance of serum response factor (SRF) and myosin heavy chain 9 (MYH9) in esophageal squamous cell carcinoma(ESCC).Methods:The clinical data of 113 patients with esophageal squamous cell carcinoma treated in Tangshan people′s Hospital from January 2014 to December 2016 were retrospectively analyzed.Immunohistochemical staining was used to detect the expression of SRF and MYH9 in esophageal squamous cell carcinoma and corresponding adjacent tissues, and the correlation between the differential expression of SRF and MYH9 and clinicopathological features was analyzed.Results:Immunohistochemical staining showed that the positive expression rate of SRF in esophageal squamous cell carcinoma was 42.48% (48/113), which was significantly higher than that of 6.25% (2/32) in adjacent tissues (χ 2=14.487, P<0.05). The positive expression rate of MYH9 was 57.52% (65/113), which was significantly higher than that of 25.00% (8/32) in adjacent tissues (χ 2=10.551, P<0.05). The expression levels of SRF and MYH9 were closely related to the differentiation, lymph node metastasis and TMN stage of ESCC (all P<0.05). The positive expression of SRF had a correlation with positive expression of MYH9( r=0.521, P<0.05). The median survival time of SRF and MYH9 double negative expression group, SRF and MYH9 single positive expression group, SRF and MYH9 double positive expression group were 30, 20 and 12 months respectively.There were significant differences between the double negative, single positive expression groups and double positive expression groups (χ 2 values were 43.855, 17.799, all P<0.001), but there was no significant difference between the double negative and single positive expression groups (χ 2=2.787, P=0.095). Conclusion:SRF and MYH9 may be one of potential targets for diagnosis and treatment of esophagus cancer.

Application of combined body position fixation in radiation therapy of lung cancer / 中华放射医学与防护杂志

Objective To explore the CTV to PTV external expansion boundary and the effect of the dose of normal lung tissue under different fixed modes by a comparative analysis of combined body position and thermoplastic film fixed set-up error of radiation therapy for lung cancer. Methods From October 2016 to March 2018, the patients who received chest radiology at the Tangshan people's hospital were enrolled as subjects retrospectively divided into two groups, including 50 patients with lung cancer radiotherapy with combined body position fixation, and 40 patients with lung cancer with thermoplastic film fixation. The two groups of patients drew the target areas in accordance with the unified standard, and the set-up error of left and right, up and down, front and rear ( x, y, z axis) were recorded respectively after 1 time/week cone CT( CBCT) matched with the planned CT image and analyzed by t test. According to the MPTV =2. 5Σ+0. 7δ, CTV to PTV external expansion boundary in the combined body position group were calculated. And the V5、V20 and V30 of two groups of patients were calculated and analyzed by TPS system. Results The set-up error of the combined body position group and thermoplastic film group were respectively (1. 00 ± 0. 58) mm and (3. 28 ± 0. 43) mm on the x axis, (1. 42 ± 0. 28) mm on the y axis and (4. 03 ± 0. 41) mm, (1. 06 ± 0. 44) mm and (3. 18 ± 0. 34) mm on the z axis. The set-up errors of the two groups were statistically significant on x, y and z axis( t= -20. 740, -35. 596, -25. 015,P<0. 05). There was no significant difference in set-up errors between the central and peripheral lung cancer patients and between left and right lung cancer patients(P>0. 05). Through the MPTV =2. 5Σ+0. 7δ, CTV to PTV external expansion boundary in the combined body position fixation group was 2. 906 , 3. 746 and 2. 958 mm on x, y and z axis respectively. The comparison between group A and B showed that the mean values of V5 , V20 and V30 in group B were reduced by 1. 5％, 3. 1％ and 4. 8％ respectively compared with group A. Conclusions The combined body position technique can improve the accuracy of lung cancer patients after radiation therapy,and further reduce the boundary of CTV to PTV, which is of certain value to reduce the occurrence of radiation pneumonitis.

Circular RNA and the relation of carcinoma / 中国综合临床

Objective Cyclic RNA ( circRNAs) was discovered in 1970s,which is different from other RNA,its structure is special,which is a closed covalent ring structure,and shows high and stable expression in eukaryotic cells?In recent years, the study found that a large number of endogenous circRNAs exists in mammalian cells,and most circRNAs with stable expression,RNA enzyme degradation resistance,usually in the tissue cells with the characteristic of diversity and specificity?CircRNAs most formed by exons,part formed by introns?For the functional study of circRNAs,circRNAs has a similar competitive endogenous RNA ( ceRNA) of the sponge function,but also can regulate transcription and translation?More and more evidence indicates that circRNAs circRNAs may be abnormal expression in many diseases,especially in tumor cells?This could be some new diagnostic and predictive biomarkers of disease?CircRNAs in the field of RNA is becoming a new research hotspot,and can be widely involved in many areas of life.

Cause of food impaction after fixed denture restoration and therapeutic effect of gap expansion and resin repair / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To discuss the cause of food impaction after fixed denture restoration and to analyze the therapeutic effect of gap expansion and resin repair.</p><p><b>METHODS</b>A total of 100 patients who suffered from food impaction after they received fixed partial denture were chosen. The cause of food impaction was analyzed. Treatment methods were applied on the basis of different causes. Gap expansion and resin repair were implemented to remove food impaction caused by the poor contact of the denture with healthy adjacent teeth.</p><p><b>RESULTS</b>The poor contact between fixed denture and healthy adjacent teeth was the major cause of food impaction (70%) . The effective rate of treatment among patients who received fixed partial denture but suffered from food impaction after 3 years was 100%.</p><p><b>CONCLUSIONS</b>The proposed method can be applied to treat food impaction caused by the poor contact of denture with healthy adjacent teeth.</p>

Expression of BTG1 protein in laryngeal squamous cell carcinoma and its clinical significance / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the expression of B-cell translocation gene 1 (BTG1) and to determine the relationship between BTG1 expression and clinicopathological features, biological behaviors in laryngeal squamous cell carcinoma.@*METHOD@#Immunohistochemistry and Western blot were used to analyze BTG1 protein expression in 70 cases of laryngeal cancer and 35 cases of adjacent corresponding laryngeal mucosal tissues to illuminate the relationship between BTG1 expression and clinical factors.@*RESULT@#The positive rate of BTG1 protein expression was 31.43% in laryngeal carcinoma tissues, significantly lower than 91.43% in the adjacent laryngeal tissues (P 0.05) of patients with laryngeal cancer.@*CONCLUSION@#The expression of BTG1 protein was decreased in laryngeal squamous cell carcinoma, suggesting that BTG1 gene may be closely associated with the carcinogenesis and the degree of malignancy. Detection of BTG1 expression may be useful in diagnosis, treatment and prognosis of laryngeal carcinoma.

The effect of BTG1 overexpression on the proliferation and apoptosis of laryngeal cancer cells and its molecular mechanismin vitro / 中国癌症杂志

Background and purpose:B-cell translocation gene 1(BTG1) can inhibit cell proliferation, promote cell apoptosis and regulate cell cycle progression and differentiation in a variety of cell types. This study aimed to explore the inlfuence on cell proliferation, apoptosis and cell cycle and its related mechanism of laryngeal cancer Hep - 2 cell lines through BTG1 overexpression byin vitro experiments.Methods:The BTG1 expression plasmids were constructed and transfected into Hep-2. They were divided into experimental group (transfected BTG1 of Hep-2 cells) and control group (transfected empty plasmid of Hep-2 cells). Western blot method was used to identify BTG1 protein expression levels of cells; proliferation activity of cells was detected by MTT assay; lfow cytometry was used to analyze the cell cycle distribution and AnnexinⅤ-FITC/PI cell apoptosis; Western blot was also used to assay cell cycle regulatory protein and apoptosis-related protein expression.Results:The pEGFP-N1-BTG1 plasmid was constructed successfully, and the expression of BTG1 protein was higher in experimental group than that in control group (0.921±0.091vs 0.308±0.047,P<0.05). Compared with the two group of laryngeal cancer Hep-2 cells, the cell growth in experimental group was slowed down and the proliferation was reduced (P<0.05); Cyclin D1 protein expression level was decreased (0.436±0.023vs 0.916±0.092,P<0.05), the proportion of G0/G1 phase cell cycle was increased [(85.1±5.2)%vs (63.8±3.1)%,P<0.05], the proportion of S phase cell was decreased [(8.3±1.1)%vs (23.1±1.5)%, P<0.05], phosphatidylserine ectropion in experimental group was increased, cell early apoptosis was significant [(10.3±1.1)%vs (2.8±0.3)%,P<0.05] and anti-apoptotic protein Bcl-2 expression level was reduced(0.167±0.009vs 0.834±0.084,P<0.05).Conclusion:BTG1 high expression could inhibit the proliferation growth of laryngeal Hep-2 cells and promote its apoptosis, and the possible mechanisms are interrelated with BTG1 involved in cell cycle regulation and causing cell apoptosis.

Clinical study of oncoplastic breast-conserving surgery for the treatment of 30 cases of early breast cancer / 中国肿瘤临床

Objective:To evaluate the clinical effects of oncoplastic breast-conserving surgery on patients with early breast can-cer near the mammary areola. Methods:A total of 60 patients with early breast cancer underwent breast-conserving surgery in the Sec-ond Department of Breast Surgery, Tangshan People's Hospital from February 2011 to November 2013. These patients were random-ized into two groups, namely, the experimental Group A (n=30) and the control Group B (n=30). Oncoplastic breast-conserving surgery was performed on the patients in Group A, whereas Group B underwent standard breast-conserving surgery. The specimen weight of the locally excised breast, the nearest distance of the tumor to the surgical margins, and the postoperative cosmetic result of the affected breast were compared between the two groups. Results: The specimen weights of the locally excised breast were 71.03 ± 12.92 and 41.53±7.13 g, and the nearest distances of the tumor to the surgical margins were 13.30±2.97 and 10.63±1.65 mm in Groups A and B, respectively, with significant differences between the two groups (P0.05). Con-clusion:A larger amount of excised breast tissue and a wider scope of surgical margins were observed in Group A patients. However, the postoperative cosmetic result of the affected breast was almost similar for both groups. Therefore, oncoplastic breast-conserving sur-gery is a feasible and effective approach for early breast cancer patients.

Correlation between serum GROβexpression and clinicopathologi-cal characteristics of colorectal cancer / 中国肿瘤临床

Objective:To confirm the potential of growth-related gene productβ(GROβ) as a biomarker for colorectal cancer. Methods:Serum GROβlevels in 123 subjects with colorectal cancer, 88 healthy controls, and 125 subjects with other diseases were measured using enzyme-linked immunosorbent assay. Serum levels of carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) in all subjects were measured using immunoluminometric assay. Statistical analyses were conducted to determine the associa-tions between serum GROβlevels and clinical parameters for colorectal cancer. The receiver operating characteristic (ROC) curves of GROβ, CEA, and CA19-9 were analyzed. Results:The serum GROβlevels were higher in patients with colorectal cancer (median=96.15 pg/mL) than in the healthy controls (median=43.28 pg/mL, P<0.01) and in patients with other diseases (median=57.30 pg/mL, P<0.01). The serum GROβlevels in patients with colorectal cancer were positively correlated with the tumor-node-metastasis staging (P<0.01) and depth of infiltration (P<0.05), but not with the histological grade, tumor embolus, lymph node metastasis, gross pathologic tu-mor type, or gender of the patients. The sensitivity and specificity of the assay for serum GROβwere 56.1%(69/123) and 95.31%(203/213), respectively. The diagnostic sensitivity was 22.2%(4/18) for stage I and 66.7%(26/39) for stage II when the data of GROβwere combined with the data of CEA and CA19-9. The ROC curve constructed with the data of GROβ(0.834) was larger than that construct-ed with the data of CEA (0.739) or CA19-9 (0.676) for discriminating colorectal cancer from the matched controls. Conclusion:These preliminary results indicated that the serum GROβlevel could be a useful biomarker for colorectal cancer diagnoses.

The research progress on antitumor function of allicin / 实用肿瘤学杂志

Allicin,which contains rich sulfide ,is provided for prevention and treatment of tumor ,anti in-flammation,prevention and treatment of cardiovascular diseases ,improving immunity and so on .Studies show that allicin can not only prevent many types of cancer ,but also has obvious inhibitory effect on tumor .The mechanism is related with antioxidant effects ,reducing the invasion of tumor cells ,inhibiting the proliferation of tumor cells , inducing tumor cell apoptosis and directly killing the tumor cells .This paper reviews the research progresses on antitumor allicin .

Efficacy and survival analysis of triple-negative breast cancer treated with neoadjuvant chemotherapy / 肿瘤研究与临床

Objective To evaluate the efficacy and survival rate of neoadjuvant chemotherapy with docetaxe and pirarubicin in triple negative breast cancer (TNBC).Methods Total 51 breast cancer patients were divided into TNBC group (n =26,including 16 of stage Ⅱ and 10 of stage Ⅲ patients) and non-TNBC group (n =25,including 14 of stage Ⅱ and 11 of stage Ⅲ patients).All patients received a median of 4 treatment cycles with TAC regimen [docetaxe 75 mg/m2 on day 1,pirarubicin 40 mg/m2 on day 1 and cyclophosphamide (CTX) 500 mg/m2 on day 1 of each 21 day cycle].The efficacy of treatment and survival rate of two groups were evaluated.Results In TNBC group,9 out of 26 (34.62 ％) patients achieved clinical complete response (cCR),and 14 (53.85 ％) had partial response (cPR).Overall,88.46 ％ of TNBC patients had clinical response and 26.92 ％ (7/26) showed pathology complete response (pCR).In non-TNBC group,6 (24.00 ％) patients reached cCR and 8 (32.00 ％) showed cPR.The overall response rate was of 56.00 ％,and 4 (16.00 ％) patients achieved pCR.The overall 3-year survival rates in TNBC and non-TNBC groups were 73.08 ％ and 88.00 ％,respectively,indicating a poorer prognosis of TNBC.The 5-year survival rates of TNBC patients with and without pCR were 88.89 ％ and 47.06 ％,respectively.Conclusion TAC regimen improves the prognosis for locally advanced TNBC,indicating that the neoadjuvant chemotherapy is effective and safe for TNBC patients.

Application of carbon nanoparticle-labeled lymph nodes in laparo-scopic curative resection for advancing rectal cancer / 中国肿瘤临床

Objective:To evaluate the clinical value of activated carbon nanoparticles for guiding lymphadenectomy in advanc-ing rectal cancer. Methods:Eighty rectal cancer patients who underwent laparoscopic curative resection for rectal cancer were divided into two groups:control group (40 cases) and experiment group (40 cases). The experiment group received carbon nanoparticle-labeled lymph nodes in surgery. The number of lymph nodes, lymph nodes≤5 mm in size, and positive lymph nodes, as well as the side effect of the procedure, were analyzed. Results:No complications were observed in the experiment group. The experiment group showed sig-nificantly higher values (P<0.05) than the control group for average number of lymph nodes (25.5 ± 8.78 vs. 16.05 ± 4.84), lymph nodes≤5mm in size (22.6 ± 8.25 vs. 13.65 ± 4.62), and positive lymph nodes (3.13 ± 4.14 vs. 1.35 ± 2.06). During operation, two dyed lymph nodes in two cases were found at the roof of the inferior mesenteric artery and along the side of the internal iliac artery. Dissec-tion was extended for these patients and the dyed lymph nodes were confirmed to be positive. Conclusion:Local injection of activated carbon nanoparticles around the tumor during surgical exploration was an effective, secure, and easy approach for guiding lymphade-nectomy in rectal cancer patients.

Jinlong capsule combined with interventional therapy for primary hepatocellular carcinomas: a clinical analysis on 150 patients / 中华肝胆外科杂志

Objective To analyze the results on patients with primary hepatocellular carcinoma who received Jinlong capsule combined with interventional therapy or interventional therapy alone.Methods During the period from March 2003 to October 2012,147 patients with primary hepatocellular carcinoma were treated in Tangshan People's Hospital with interventional therapy.These patients were randomly divided into two groups:combined interventional group (n=74) and interventional alone group (n=73).Results Compared with the interventional therapy alone group,the efficacy,quality of life,and the liver function using the Child-Pugh classification were significantly better in the combined interventional therapy group for tumor less than 5 cm (P＜0.05) than the interventional alone group.The combined interventional therapy group was also significantly better than the interventional alone group in WBC count (P＜0.05).Conclusion Jinlong capsule combined with interventional therapy was superior to interventional therapy alone for patients with primary hepatocellular carcinoma,especially when the tumor was less than 5 cm.

Relationship between Survivin protein and esophageal cancer: a systematic review / 肿瘤研究与临床

Objective To investigate Survivin protein and esophageal cancer with a systematic review.Methods The published studies were searched in the Cochrane Library (Issuel,2011),Pubmed and CNKI databases, and other relevant journals were also hand searched to identify all the relevant case-control trials.Then the quality of the included trials was assessed and meta-regression analysis was performed,by STATA 11.0 software.Results A total of 15 studies were recruited.As for the positive rate of Survivin expression, significant differences were tested between esophageal cancer vs.normal esophageal tissues (OR =39.98, 95 ％ CI =15.08-106.02, P ＜ 0.05).No significant difference was tested between cell differentiation G3 vs.cell differentiation G1-G2(OR =1.47,95 ％ CI =0.59-3.68,P ＞ 0.05).There were significant differences between T stagesⅢ-Ⅳ vs.T stages Ⅰ-Ⅱ (OR =2.82,95 ％ CI =1.35-5.90,P ＜ 0.05),clinic stages Ⅲ-Ⅳ vs.clinic stages Ⅰ-Ⅱ (OR =2.99, 95 ％ CI =2.03-4.41, P ＜ 0.05), lymph node metastasis vs.non-lymph node metastasis (OR =4.11,95 ％ CI =2.79-6.05,P ＜ 0.05).Moreover,Survivin expression was positively correlated with the expression of hypoxia inducible factor-1α (HIF-1α) (r =0.542,P ＜ 0.005).Conclusion Survivin expression increased malignant behaviors of esophageal cancer.There might be substantial correlations between Survivin and HIF-1α expression and esophageal cancer.

Nebivolol in the Inhibition of eNOS Activity in Cadiocytes with or without Heart Failure / 中国药房

0.1), but which were inhibited significantly in the left ventricular cardiac myocytes of the subjects with heart failure(P≤0.05). Both carvedilol and metoprolol exhibited no effect on eNOS activity in all the investigated cardiac myocytes. CONCLUSIONS: Nebivolol does no effect on eNOS activity of left ventricular cadiocytes in subjects or rats without hear failure but it can inhibit eNOS activity of cadiocytes in subjects or rats with heart failure so as to exert its beneficial clinical effect.

Preoperative diagnostic value of ~(131)I-SPECT/CT fusion images in detecting the cervical and superior mediastinal metastases of DTC / 中国耳鼻咽喉头颈外科

OBJECTIVE To investigate the diagnostic value of 131I-SPECT/CT fusion images in detecting the metastases of differentiated thyroid carcinoma(DTC)in the regions of the head,neck and superior mediastinum.METHODS 131I-SPECT/CT fusion images was preformed for 30 patients with DTC,and the results of image were compared with the pathology after surgery.RESULTS There were 110 cases positive findings in 115 metastatic lesions with 131I-SPECT/CT fusion images examination(95.65%).5 cases of the metastatic lesions showed negative in 131I-SPECT/CT(4.35%).There was no false positive case.CONCLUSION 131I-SPECT/CT fusion images can detect and locate the metastasis and exclude the false positive results accurately.It is a good method to detect the cervical and superior mediastinal metastatic lesion in differentiated thyroid carcinoma.

Effects of different CO_2 pressure on duration of pneumoperitoneum and shoulder pain following laparoscopic cholecystectomy / 中国微创外科杂志

0.05). The stepwise regression analysis showed that the logarithm of the duration of residual gas had a parallel relationship with the gas volume under the hemi-diaphragm 24 hours after operation(r=0.616,P=0.006),a negative correlation with the duration of pneumoperitoneum (r=-0.228,P=0.014), and no correlation with patients’ age,body weight,height,operation time and gas volume consumed. Postoperatively, 26 patients reported postoperative shoulder pain. The shoulder pain VAS scores were significantly increased with the increasing of CO_2 pressure in the three groups (F=9.635, P=0.000). The shoulder pain VAS scores had a parallel relationship with the gas volume under the right hemi-diaphragm 24 hours after operation (r=0.333, P=0.005) and the duration of residual gas (r=0.296, P=0.014). Conclusions The CO_2 should be removed as thoroughly as possible at the end of operation to reduce the absorption of residual gas. The different CO_2 pressures of pneumoperitoneum have little effects on the volume of residual CO_2 24 hours after operation and the duration of residual gas. The shoulder pain following LC, will be aggravated with the increasing of pneumoperitoneum pressure. The over-stretching of the diaphragmatic fibers due to insufflation is the main cause of shoulder pain.

Ethical Analysis of Immoderate Medication in Clinical Practice / 中国医学伦理学

Immoderate medication is a common phenomenon in medical institutions with very complicated causes.Intervention measures should be employed in order to relieve the burden on patients and realize the rational use of drug.Therefore,it remains a highly complex and difficult task before the healthcare administrators.The improvement must be accomplished through moral education for medical staff and concentrating on institutional improvement,thus the patient-oriented creed of medical institutions could be truly incarnated.